临床医学论文-聚酮合成酶底物专一性的研究进展

临床医学论文-聚酮合成酶底物专一性的研究进展聚酮合成酶底物专一性的研究进展 【摘要】 聚酮合成酶能够合成包括许多大环内酯类抗生素在内的聚酮类天然产物，它的结构和功能的模块性为组合生物合成的产生和发展奠

临床医学论文-聚酮合成酶底物专一性的研究进展 聚酮合成酶底物专一性的研究进展 【摘要】 聚酮合成酶能够合成包括许多大环内酯类抗生素在内的聚酮类天然 产物，它的结构和功能的模块性为组合生物合成的产生和发展奠定了基础。聚 酮合成酶的酰基转移酶功能域可以特异性地决定所选择酰基辅酶A的种类，决 定产物的结构。近年来，针对许多来源不同、结构各异的聚酮合成酶的底物专 一性已经从氨基酸序列、结构和功能等方面进行了大量的研究，为更有效地应 用聚酮合成酶开发新型抗生素奠定了基础。 【关键词】 聚酮合成酶; 底物专一性; 组合生物合成; 酰基转移酶 Progress in substrate specificity of polyketide synthase ABSTRACT Polyketide synthases catalyze the synthesis of complex natural products including macrolide antibiotics from simple structural and functional modularity of these multienzyme systems has raised the possibility that polyketide biosynthetic pathways might be rationally reprogrammed by combinatorial manipulation. Previous studies have shown that the choice of these substrates is primarily controlled by individual acyltransferase (AT) domains within each PKS harnessing this biosynthetic potential is abetter understanding of the molecular recognition features of polyketide synthases. Within this decade, a variety of genetic, biochemical, and chemical investigations have yielded insights into the specificity of several architecturally different polyketide synthases. The results of these studies, together with their implications for biosynthetic engineering, are summarized in