大鼠垂体前叶内神经纤维对LH分泌的兴奋性调节作用

大鼠垂体前叶内神经纤维对LH分泌的兴奋性调节作用　　【关键词】 电场刺激；黄体生成素；垂体前叶；神经纤维；卵巢切除术；河豚毒素；大鼠　　Excitatory regulative effect of

大鼠垂体前叶内神经纤维对LH分泌的 兴奋性调节作用 【关键词】 电场刺激；黄体生成素；垂体前叶；神经纤维；卵 巢切除术；河豚毒素；大鼠 Excitatory regulative effect of nerve fibers on LH secretion in rat anterior pituitary 【Abstract】 AIM: To investigate the direct regulative effect of nerve fibers on luteinizing hormone （LH） secretion in rat anterior pituitary. METHODS: Eighty diestrus rats determined by vaginal smear were randomly divided into sham operation (SO) and ovariectomy (OVX) groups. Seven days after operations, the anterior pituitary was harvested and superfused with Krebs solution (KrebsRingerbicabonateBSA buffer, KRBGA) with or without tetrodotoxin (TTX). Electrical field stimulation (EFS) was performed at 10 Hz or 2Hz for 10 min or 2min, respectively, and the perfusate was continuously collected every 10 min for 130 min from the time 10 min before EFS and measured for LH by radioimmunoassay. RESULTS: In the anterior pituitary superfused with KRBGA without EFS, LH