氧化型低密度脂蛋白对骨髓内皮前体细胞增殖凋亡及NO分泌功能的

氧化型低密度脂蛋白对骨髓内皮前体细胞增殖、凋亡及NO分泌功能的　　作者：宗小娟，王海昌，周祥，程康 【关键词】 内皮祖细胞 Effect of oxidized lowdensity lipoprot

氧化型低密度脂蛋白对骨髓内皮前体细 胞增殖、凋亡及NO分泌功能的 作者：宗小娟，王海昌，周祥，程康【关键词】内皮祖细胞 Effectofoxidizedlowdensitylipoproteinon proliferation,apoptosis,andNOsecretingfunctionof endothelialprogenitorcellsfrombonemarrow 【Abstract】AIM:Tostudytheeffectofoxidized lowdensitylipoprotein(oxLDL)ontheproliferation, apoptosis,andNOsecretingfunctionofendothelial progenitorcells(EPCs).METHODS:BonemarrowderivedEPCs wereisolatedfrombonemarrowbloodofswinebydensity gradientcentrifugation,thenoxLDLofdifferent concentrations(groupB,5mg/L;groupC,10mg/L;groupD, 20mg/L)wereaddedintoculturemediumtodetectthe effectofoxLDLonEPCs.MTTassaywasusedtodetectthe effectofoxLDLonthemultiplicationabilityofEPCs.Flow cytometrywasusedtodetecttheapoptosiscausedbyoxLDL. ThenNOcontentwasmeasuredinthecellculturemediumby nitratereductasemethodtofindtheinjuryofoxLDLon