幽门螺杆菌对抗生素的敏感性及细胞毒素相关蛋白A基因检测

打L力修床消化消化* 20W)'年第12卷第5期・论 著・幽门螺杆菌对抗生素的敏感性及细胞毒素相关蛋白A基因检测 我方冰、2上海第二医科大学微生物教研室(上海2000251吴红手洪涛时晓东刘晶星摘 要

・・ 195 打L力 ) 20W'125 修床消化消化* 年第卷第期 ・・ 论著 A2 幽门螺杆菌对抗生素的敏感性及细胞毒素相关蛋白基因检测我方冰、 2000251 上海第二医科大学微生物教研室(上海吴红手洪涛时晓东刘晶星 1S3URA ("A) 摘要 目的：检测株幽螺杆菌对甲硝理、因讣片.可莫西林的敏感性内俄胺的和细胞毒京相关黄白基因。方法:琼脂稀 PCR3 A72.5%, 释法检测药物最能抑前法度、纸片假度定法检测伊内酰胺摩及法检测基因。 结果：甲硝理耐药前株为四环水耐药菌株为 ( 58.9%,41.2%31%P<0.05)c 阿英西林耐药菌株为。三重耐药菌株达。来 自女性的甲硝噬耐药浦煤比来自男性的多见所有菌侏缶内酰胺除均 ( Cag A66.7%£Cag AP<0.05)HP 阴性。含基因的菌株为、 井发现甲硝嗖依照阙株基因比耐西菌株更常见。结论：本地区耐药菌株多见，且 出现大量多重 耐药菌株。网英西林耐药机制与小内院胺院产生无关。耐药性与希力基因存在一定相关性。 叼 cR A 关键词吧咚杆菌啰"敬照仕细胞褥取相关蛋白基囚用内酰脓■ Detection of Antibiotic Susceptibility and Cytotoxin Associated Protein AGene in Helicobacter Pylori ( Wu 11Wang l<ong-t»o. Shi Xiao-dong et al 鹏、 IHpartment ot Microbiology, Shanghai Second Medical University ： Abstracts Purpose To investigate the Helicobacter pylori (HP) antibiotic resistance to metronidazole, tetracycline, ： amoxycillin; 3"lactamasc and Cytotoxin Associated Protein A(Cag A) gene. Methods 153 helicobacter pylori clinic isolates collected from the number nine People's hospital affiliated to Shanghai second medical university during the period from January 1998 to February 1999 were examined. Minimum inhibitory concentration]M(') was detected by agar ： dilution method, "lactamase by means of acidometry using “actamase detection paper and cag Agene by PCR. Results 72. 5% of strains was resistant to metronidazole, 58.9% was resistant to tetracycline and 41.2% was resistant to amoxycillin. Multiresistant strains which resist to the three antibiotics in the meantime were prevalent (31%). Metronidazole resistance were more common in female than in male( P< 0. 005). p-lactamase of all strains was negative. 66.7% of isolates had Cag Agene. Surprisingly, metronidazole sujceptible strains with Cag Agene were more ： frequent than resistant strains (P <0. 05). Conclusion This study shows not only the distribution of antibiotic resistance and Cag Agene, but also acertain relationship between them. The mechanism of amoxycillin resistance is not linked to the production of P-lactamase. This study may be helpful in the epidemiological survey as well as control of helicobacter pylori infections in this area. Key word Helicobacter pylori Antibiotic susceptibility Cyroroxin associated protein Agene 0-lactamase (H&cobacter pylori, HP) 幽门螺杆菌常引起慢性 目 HP 行了检测。为探讨阿英西林耐药机制，及中细胞毒 炎、消化性溃疡，也与胃癌和胃淋巴病的发生密切相 关， A, A(Cytotoxin associated protein Ca& A) 素相关蛋白 基 HP 治疗感染常用以抗生素为主的三联疗法。最 常用的 因与抗生素耐药性是否存在一定关系，故 同时检洲自 c 抗生素为甲硝喋、克拉霉素、阿莫西林、四环索但复 Cag A 内酰胺酶产生和基因。 发常见。治疗失败的主要原因是抗生素耐药菌株 的产 生。因此定期对抗生索敏感性的监测不仅可了解 本地 1 材料和方法 HPHP 区耐药状况,而且可指导临床用药，故本试验 就 对常用的抗生素甲硝嘤、阿莫西林、四环索的耐 药性进 11153HP199811999 2 菌株株菌株来自年月至年