Glucose GOD-PAP - Diatek：葡萄糖- diatek GOD-PAP

Glucose GOD Diagnostic reagent for quantitative in vitro determination of glucose in serum or pla__a

GlucoseGOD Diagnosticreagentforquantitativeinvitrodeterminationofglucoseinserumorpla__aonphotometric systems WarningsandPrecautions OrderInfor__tion 1. Thereagentcontainssodiumazide(0.95g/l)as preservative.Donotswallow!__oidcontactwithskinand CatalogNo Kitsize mucousmembranes. 2. Takethene__ssaryprecautionsfortheuseoflaboratory D1G25–125 5x25ml reagents. D1G25–150 6x25ml D1G25–300 12x25ml D1G25–500 1x500ml D1G25–600 6x100ml 1000ml D1G25–LT1 Sum__ry [1,2] Measurementofglucosecon__ntrationinserumorpla__ais __inlyusedindiagnosisandmonitoringoftreatmentin diabetesmellitus.Otherapplicationsarethedetectionof neonatalhypogly__mia,theexclusionofpancreaticislet__ll carcino__aswellastheevaluationofcarbohydratemetaboli__ invariousdiseases. Method “GOD-PAP“:enzy__ticphotometrictest Principle Determinationofglucoseafterenzy__ticoxidationbyglucose oxidase.Thecolorimetricindicatorisquinoneimine,whichis generatedfrom4-aminoantipyrineandphenolbyhydrogen peroxideunderthecatalyticactionofperoxidase(Trinder’s reaction)[3]. GOD Glucose+O >Gluconicacid+HO 222 POD 2HO+4-Aminoantipyrine+Phenol >Quinoneimine+4HO 222 Reagents ComponentsandCon__ntrationsintheTest Reagent: Phosphatebuffer pH7.5 250mmol/l Phenol 5mmol/l 4-Aminoantipyrine 0.5mmol/l Glucoseoxidase (GOD) 10kU/l Peroxidase (POD) 1kU/l Standard: 100mg/dl(5.55mmol/l) StorageInstructionsandReagentStability Thereagentisstableuptotheendoftheindicatedmonthof expiry,ifstoredat2–8°C,protectedfromlightand contaminationis__oided.Donotfreezethereagents! Note: Ithastobementioned,thatthemeasurementisnot influen__dbyoccasionallyoccurringcolorchanges,aslongas theabsorban__ofthereagentis<0.3at546__. Thestandardisstableuptotheendoftheindicatedmonth ofexpiry,ifstoredat2–25°C. 2004/05Rev:00GlucoseGOD–Page1