拟南芥miR156上游调控因子的鉴定

拟南芥miR156上游调控因子的鉴定Title: Identification of Upstream Regulators of miR156 in Arabidopsis thaliana Abs

miR156 拟南芥上游调控因子的鉴定 Title: Identification of Upstream Regulators of miR156 in Arabidopsis thaliana Abstract: MicroRNAs (miRNAs) are crucial regulators of gene expression, playing essential roles in various biological processes in plants. In Arabidopsis thaliana, miR156 has been found to be akey regulator of the juvenile-to-adult phase transition and other developmental processes. However, the upstream regulators that control the expression of miR156 in Arabidopsis remain largely unknown. In this study, we aimed to identify the upstream regulators of miR156 and investigate their potential roles in regulating the miR156 pathway. Introduction: MicroRNAs are small non-coding RNAs that negatively regulate gene expression by targeting mRNA transcripts for degradation or translational repression. miR156 is one of the most conserved miRNAs among plants and is known to play acrucial role in the transition from the juvenile to the adult phase in Arabidopsis thaliana. However, the upstream regulators that control the expression of miR156 in Arabidopsis are not well characterized. Methods: To identify potential upstream regulators of miR156, we employed an integrated bioinformatics approach. Firstly, we performed a promoter analysis of the miR156 gene to identify putative cis-regulatory elements. We then used publicly available microarray data to identify transcription factors that are co-expressed with miR156. Subsequently, we examined the binding sites of the identified transcription factors in the miR156 promoter region. Finally, we validated the regulatory roles of the identified transcription factors through gene expression analysis and functional assays. Results: Our analysis revealed several putative cis-regulatory elements in the promoter region of the miR156 gene, suggesting that the expression of miR156 is regulated by transcription factors. By mining publicly available microarray data, we identified several transcription factors that are co-expressed with miR156, including SPL family