西兰花端粒酶分离、检测技术研究

西兰花端粒酶分离、检测技术研究Abstract:Broccoli is a popular vegetable known for its high nutritional value and pot

西兰花端粒酶分离、检测技术研究 Abstract: Broccoliisapopularvegetableknownforitshighnutritional valueandpotentialhealthbenefits.However,post-harvestquality deteriorationandrapidsenescencearemajorchallengesin broccoliproductionandmarketing.Oneofthemechanisms underlyingtheseprocessesistheactivityoftelomerase. Telomeraseisaribonucleoproteincomplexresponsiblefor themaintenanceoftelomeres,theprotectivecapsattheendof linearchromosomes.Itsactivityisgenerallylowinsomaticcells, leadingtoprogressivetelomereshorteningandeventuallycell deathorsenescence.Recentstudieshavesuggestedalink betweentelomeraseactivityandpost-harvestqualityof vegetables,includingbroccoli. Inthisreview,wesummarizethecurrentunderstandingof telomerasebiologyandfunctioninplants,withafocuson broccoli.Wealsodiscussvarioustechniquesformeasuring telomeraseactivityandtheirapplicationinthestudyofbroccoli physiologyandqualitycontrol.Finally,wehighlightthepotential ofmanipulatingtelomeremaintenancepathwaysforimproving post-harvestqualityandextendingshelflifeofbroccoli. Introduction: Broccoli(Brassicaoleraceavar.italica)isavegetable belongingtotheBrassicaceaefamily,whichalsoincludescabbage, cauliflower,andkale.Itisrichinvitamins,minerals,dietaryfiber, andantioxidants,andhasbeenlinkedtovarioushealthbenefits, suchasreducingtheriskofcancer,cardiovasculardisease,and inflammation(Podsędek,2007;TalcottandSchmitz,2012).As such,broccoliisapopularingredientinmanycuisinesanda valuablecommodityintheglobalvegetablemarket. However,thepost-harvestqualityandshelflifeofbroccoliare limitedbyseveralfactors,includingphysicaldamage,microbial growth,andsenescence.Senescenceisacomplexprocess involvingvariousbiochemicalandphysiologicalchangesthatlead tothedeclineinqualityandnutritionalvalueoftheharvested produce.Oneofthehallmarksofsenescenceistelomere shortening,whichactivatescellcyclecheckpointsandtriggers