T淋巴瘤侵袭转移诱导因子1与胃癌细胞侵袭移行能力的关系
T淋巴瘤侵袭转移诱导因子1与胃癌细胞侵袭移行能力的关系 毕业论文 &nbs
T1 淋巴瘤侵袭转移诱导因子与胃癌细胞侵袭移行能力的关系 毕业论文            作者：朱金明余佩武 李翠芳吴淼   T1(T lymphoma invasion 【摘要】目的检测胃癌细胞中淋巴瘤侵袭转移诱导因子 and metastasis inducing factor 1Tiam1) ，的表达及其与肿瘤侵袭、移行能力的关系，同时观 MKN45 察胃癌细胞在形态学方面的变化。方法采用层黏连蛋白黏附法，由胃癌细胞株 (M0)(MH)(ML)Tiam1 中筛选获得高、低黏附亚株。应用流式细胞仪检测在胃癌细胞中的表 BoydenM0MLMHTiam1 达，以小室法测定、、细胞的体外侵袭、移行能力，并分析其与 表达间的关系，同时采用苏木精伊红及细胞骨架蛋白染色、扫描电镜技术观察胃癌细胞形 MHTiam1M0ML(P<0.05) 态。结果细胞的体外侵袭、移行能力及表达均强于、细胞， Tiam1(r=0.9971.000)M0ML 且表达与胃癌细胞体外侵袭、移行能力呈正相关和；同时与、 MH 相比，胞体伸展，表面突起增多，片状伪足宽厚，丝状伪足细长而密集，异型性明显， M0ML 且细胞骨架结构较紊乱，斑点状肌动蛋白小体增多。但、细胞间差异无统计学意义 (P>0.05) Tiam1 。结论表达水平升高可能促进胃癌细胞侵袭、移行。这或许是通过调整 胃癌细胞骨架结构重组，增强其变形、游走能力而实现的。   T1 【关键词】胃癌；淋巴瘤侵袭转移诱导因子；侵袭；移行；形态学 Correlation between invasion and migration ability of gastric cancer cells and Tiam 1    Abstract  Objective  To investigate the expression of Tlymphoma 【】 invasion and metastasis inducing factor 1(Tiam1) in gastric cancer cells and its relationship with the invasion and migration ability of gastric cancer cells, and observe the morphological changes of gastric cancer cells. Methods  High adhesive subgroups (MH) and low adhesive subgroups (ML) were separated from MKN45 cell strain (M0) by laminin adhesion method in vitro. The expressions of Tiam1 in M0, ML and MH cells were detected by flow cytometry, the invasion and migration ability of M0, ML and MH cells in vitro by Boyden chamber. The correlation between invasion and migration ability of M0, ML, MH cells and expression of Tiam1 in M0, ML, MH cells was analyzed. The morphological differences among M0, ML and MH cells were observed by hematoxylin eosin stain, cytoskeletal protein stain and scanning electronic microscope. Results  The invasion and migration ability and the expression of Tiam1 in MH cells were higher than those in M0 and ML cells (P<0.05). The expression of Tiam1 was positively correlated with the invasion and migration ability of gastric cancer cells (r=0.997, 1.000). Compared with the M0 and ML cells, the MH cell bodies elongated with wide and thick
