6BA对中国石竹雄性不育植株离体保存的影响
6-BA对中国石竹雄性不育植株离体保存的影响chinensis.Theculture.The株带芽茎段为初始外植体进行组织培养,筛选了适合其芽诱导、继代增殖的培养基配方,并对试管苗移栽介质进行了挑选,
6-BA对中国石竹雄性不育植株离体保存 的影响 chinensis.Theculture.The株带芽茎段为初始外植体进行组织培 养,筛选了适合其芽诱导、继代增殖的培养基配方,并对试管苗移栽 介质进行了挑选,成功地建立了2个中国石竹雄性不育植株的离体繁 殖与保存体系。结果表明:中国石竹芽的最佳诱导培养基为MS+6-BA 1.0mg/L+NAA0.3mg/L,最佳继代增殖培养基为MS+6-BA0.3 mg/L+NAA0.1mg/L,最适宜的试管苗移栽基质是珍珠岩。同时,2 个不育材料组织培养繁殖后代仍表现为雄性不育,确保了原材料的雄 性不育性状的稳定性。 关键词中国石竹;6-BA;离体繁殖;雄性不育 AbstractStemnodalsegmentsofmalesterileplantsof twocultivarswereusedasthefirstexplantsfortissue cultureandmicropropagationofDianthuschinensis.The objectsofthestudyweretoselecttheoptimummediumfor adventitiousbudinduction,proliferationandtransplanting, andeventuallyestablishthetechniquesystemoftissue culture.Theresultsshowedthattheoptimummediumfor adventitiousbudinductionandproliferation respectively.Theperlite.Then3mg/L,MS+6-BA0.3mg/L+NAA0.1 mg/L,respectively.Theoptimumtransplantingsubstratewas

