372_HPLC法测定木豆叶中牡荆苷的含量荆苷的含量

372_HPLC法测定木豆叶中牡荆苷的含量荆苷的含量HPLC法测定木豆叶中牡荆苷的含量 12曾彩芳，肖佳尚(1.广州市黄浦港湾医院药剂科，广州 510700;广州市药品检验所三分所，广州 510700

372_HPLC法测定木豆叶中牡荆苷的含量荆苷的含量 HPLC法测定木豆叶中牡荆苷的含量 12曾彩芳，肖佳尚(1.广州市黄浦港湾医院药剂科，广州 510700;广州市药品 检验所三分所，广州 510700) 摘要:目的 建立木豆叶中牡荆苷的含量测定方法。方法 采用HPLC法测定牡荆 苷的含量，色谱柱:Nucleodur C18 Gravity柱(250 mm×4.6mm，5μm);流动相:甲 醇-1%冰醋酸(梯度洗脱);流速:1.0mL/min;检测波长:339nm。结果 牡荆苷在 0.108~0.541μg范围内具有良好的线性关系，r=0.9999，平均回收率为97.79%， RSD为1.35%。结论 该方法准确、可靠，可用于木豆叶的质量控制。 关键词:木豆叶;牡荆苷;高效液相色谱法 Determination of Vitexin in Folium Cajani by HPLC Zeng Cai-fang(The frist Hospital of Guangzhou HanugPu GangWang， Guangzhou 510405,china 510500) Abstract: Objective To establish amethod for the determination of in Folium Cajani by HPLC. Method HPLC with Hypersil ODS(250×4.6mm，5μm) column was used, the 2 Methanol-1%Acetic Acid Glacial(gradient elution) as amobile phase and detection wavelength at 339nm.Results The linear range of Vitexin was from 0.11 to 0.55μg,γ=0.9999.The average recovery was 97.79%，and RSD was 1.35%。Conclusion This method is effective and