荞麦内转录间隔区(ITS)的扩增及序列分析-四川农业大学

荞麦内转录间隔区（ITS）的扩增及序列分析生物科学2002级 曾子贤指导老师 吴 琦 副教授摘 要：本研究以野生金荞麦、栽培苦荞为材料，采用改进的CTAB法提取荞麦总DNA。设计一对特异性引物，对

1 16 第页共页 编号： 2021xx 时间：年月日 页码：第页共页 1 16 书山有路勤为径，学海无涯苦作舟 ITS 荞麦内转录间隔区（）的扩增及序列分析 2002 生物科学级曾子贤 指导老师吴琦副教授 摘要： CTABDNA 本研究以野生金荞麦、栽培苦荞为材料，采用改进的法提取荞麦总。 ITSPCR700bp 设计一对特异性引物，对三份荞麦区序列进行扩增，均获得长约的单一条 PCR1699bpITS 带。产物测序结果表明，野生金荞麦号测序片段为，包含区序列为 660bp2678bpITS646bp ；野生金荞麦号测序片段为，包含序列为；苦荞测序片段为 640bpITS599bpITSGenBank ，包括序列为。将所得到的三份荞麦区序列分别与中登录 ITS1ITS90.8% 的荞麦序列进行比较。结果表明，野生金荞麦号与已知金荞麦区同源率为， 2ITS91.6%ITS 野生金荞麦号与已知的金荞麦区序列同源率达到。供试苦荞与已知苦荞 99.8% 区同源率为。 关键词： ITS5.8S rDNAPCR 野生金荞麦；苦荞；内转录间隔区（）序列；；；序列分析 Amplification and Sequences analysis of Internal Transcribed Spacer of Buckwheat ZENG Zi-xian Biological Science, Grade 2002 Directed by WU Qi (Associate Prof. Ph. D) F. cymosum F. tataricum Abstract Wildness and cultivated were investigated in this ： article. By the improving method of CTAB DNA extraction, total DNA from three buckwheat was obtained. Apair of specific primers was designed. The ITS regions of three buckwheat were amplified. The amplified fragments, about 700bp in length, were sequenced directly. The results show that the sequencing fragments are 699bp, 678bp and F. cymosumF. tataricum 640bp in length, including ITS regions of and are 660bp, F. cymosumF. tataricum 646bp and 599bp in length. Comparing and with sequences F. cymosum reported, it suggests that the homology rate of ITS sequence of ,NO. 1is