感受态细胞制备质粒DNA转化和提取
感受态细胞的制备—LB液体培养基:胰蛋白胨,1%(W/V);酵母提取物,0.5%(W/V);NaCl,1%(W/V),用固体NaOH调节pH为7.0~7.2,分装于三角瓶中,灭菌后存于室温。摇动三角瓶
感受态细胞制备质粒DNA转化和提取