芒果苷小檗碱盐在HepG2细胞中调节糖脂代谢的作用研究

芒果苷小檗碱盐在HepG2细胞中调节糖脂代谢的作用研究Title: The Effect of Mangiferin and Berberine Salt on the Regulation of G

HepG2 芒果苷小檗碱盐在细胞中调节糖脂代谢的作 用研究 Title:TheEffectofMangiferinandBerberineSaltonthe RegulationofGlucoseandLipidMetabolisminHepG2Cells Abstract: Withtheincreasingprevalenceofmetabolicdisorderssuchas diabetesandobesity,thereisagrowingneedforeffective therapeuticagentsthatcanregulateglucoseandlipid metabolism.Thisstudyaimstoinvestigatethepotentialof mangiferinandberberinesaltinregulatingthesemetabolic processesinHepG2cells.HepG2cellsweretreatedwithvarious concentrationsofmangiferinandberberinesalt,andtheireffects onglucoseandlipidmetabolismmarkerswereassessed.The resultsshowedthatbothmangiferinandberberinesalt significantlydecreasedglucoseuptakeandglycolyticactivityin HepG2cells.Furthermore,bothcompoundsincreasedinsulin sensitivityandpromotedtheactivationofAMP-activatedprotein kinase(AMPK),akeyregulatorofcellularenergyhomeostasis. Additionally,mangiferinandberberinesalttreatmentresultedina significantreductioninlipidaccumulationandtriglyceridelevels inHepG2cells.Thesefindingssuggestthatmangiferinand berberinesalthavepotentialastherapeuticagentsforthe treatmentofmetabolicdisorders. 1.Introduction: Metabolicdisorderssuchasdiabetesandobesityaremajor healthconcernsworldwide,leadingtoincreasedmorbidityand mortalityrates.Dysregulationofglucoseandlipidmetabolismisa crucialfactorcontributingtothedevelopmentandprogressionof theseconditions.Currenttherapeuticstrategiesforthetreatment ofmetabolicdisordersoftenhavelimitationsandsideeffects. Hence,thereisaneedfornoveltherapeuticagentsthatcan effectivelyregulateglucoseandlipidmetabolism.Plant-derived bioactivecompounds,suchasmangiferinandberberinesalt,have shownpromisingpotentialinthisregard. 2.MaterialsandMethods: 2.1Cellculture:HepG2cellswereculturedinDulbecco's