水酶法提取山桐子油工艺研究
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水酶法提取山桐子油工艺研究 摘要:以山桐子为原料,利用水酶法提取山桐子油。首先从纤维素酶、碱性蛋白酶、淀粉 酶、复合酶中选取提取山桐子油的最佳用酶。试验结果表明纤维素酶对提取山桐子油的作用 61.28% 最佳,其提油率达到。再通过单因素和正交试验,分别研究了料液比、加酶量、酶 ... 解 <p>&nbsp; 摘要:以山桐子为原料,利用水酶法提取山桐子油。首先从纤维素酶、碱性蛋 白酶、淀粉酶、复合酶中选取提取山桐子油的最佳用酶。试验结果表明纤维素酶对提取山桐 61.28% 子油的作用最佳,其提油率达到。再通过单因素和正交试验,分别研究了料液比、 1﹕4 加酶量、酶解时间、酶解温度对提油率的影响。获得的最佳工艺条件为料液比,加酶 2.5%5h50℃68.96%<br/> 量,酶解时间,酶解温度,此时的提油率达到。 <br/> 关键词:山桐子;水酶法;纤维素酶;提油率 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;& nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nb sp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br/> AqueousEnzymaticExtractionOilOfIdesiaPolycarpaMaxim<br/> Abstract:ThispaperreportedthestudythatusedIdesiapolycarpaMaximseedsasrawmaterials toextracttheseedoilbyaqueousenzymaticmethod.First,toselecttheoptimumenzymeof extractingIdesiapolycarpaMaximoilbyscreeningcellulose,alkalineprotease,amylaseand complexenzyme(cellulose:alkalineprotease:amylase=1:1:1),Theresultshowedthatthe cellulaseisthebestoneamongthefourkindsofenzyme,theoilextractionratereachedto 61.28%.Throughsinglefactorsandorthogonalexperiments,theeffectsoftheratioofsolidto liquid,enzymesaddition,enzymolysistimeandenzymolysistemperaturetooilextractionrate werestudied.Theoptimumconditionwasobtainedasfollows:theratioofsolidtoliquidis1︰4, enzymeadditionis2.5%,enzymolysistimeis5handenzymolysistemperatureis50℃,underthis conditiontheoilextractionratereachedto68.96%.<br/> Keywords:IdesiapolycarpaMaxim;Aqueousenzymaticmethod;Cellulose;Oilextraction rate<br/>

