METTL3介导m6A修饰对断奶仔猪F18大肠杆菌抗性的调控机制分析

METTL3介导m6A修饰对断奶仔猪F18大肠杆菌抗性的调控机制分析Title: Regulatory Mechanism of METTL3-mediated m6A Modification on

METTL3m6AF18 介导修饰对断奶仔猪大肠杆菌抗 性的调控机制分析 Title:RegulatoryMechanismofMETTL3-mediatedm6A ModificationontheResistanceofF18EscherichiacoliinWeaning Pigs Abstract: Thepost-transcriptionalmodificationofmessengerRNA (mRNA)playsacrucialroleinregulatinggeneexpressionandhas beenassociatedwithvariousphysiologicalprocessesanddisease states.N6-methyladenosine(m6A)modification,catalyzedbythe METTL3methyltransferase,isthemostabundantandconserved modificationineukaryoticmRNA.Emergingevidencesuggests thatm6Amodificationisinvolvedintheresponsetobacterial infectionandtheregulationofhostdefense.Inthisstudy,we aimedtoanalyzetheregulatorymechanismofMETTL3-mediated m6AmodificationontheresistanceofF18Escherichiacoliin weaningpigs. Introduction: Weaningisacriticalperiodinthelifeofpigsthatis associatedwithincreasedsusceptibilitytoentericdiseases,suchas infectionscausedbyF18Escherichiacoli.Understandingthe molecularmechanismsunderlyinghost-pathogeninteractions duringthisperiodisofgreatimportanceforimprovingpighealth andproductivity.Previously,m6Amodificationhasbeenshownto modulateviralinfectionoutcomesandinnateimmuneresponses. However,theroleofm6Amodificationinbacterialinfections, particularlyinweaningpigs,remainslargelyunexplored. Methods: Weusedaweaningpigmodeltoinvestigatetheregulatory mechanismofMETTL3-mediatedm6Amodificationonthe resistanceofF18Escherichiacoli.Pigletswereorallyinfectedwith F18Escherichiacoli,andintestinaltissuesampleswerecollectedat specifictimepointsforfurtheranalysis.RNAsequencing (RNA-seq)wasperformedtoexaminethemRNAprofilechanges inducedbyF18Escherichiacoliinfection.TheMETTL3-mediated m6Amodificationwasassessedthroughimmunoprecipitation,