丹参酮A对谷氨酸联合淀粉样蛋白诱导细胞损伤的保护作用临床医学论文

丹参酮ⅡA对谷氨酸联合淀粉样蛋白诱导细胞损伤的保护作用_临床医学论文 作者：张萌涛 钱亦华 杨杰 史利利 朱淑娟 刘朝晖【摘要】 目的 探讨丹参酮ⅡA(TanⅡA)对谷氨酸(Glu)联合β淀粉样蛋白2

丹参酮ⅡA对谷氨酸联合淀粉样蛋白诱导细胞损伤的保护作用_临床医学论文 作者：张萌涛 钱亦华 杨杰 史利利 朱淑娟 刘朝晖 【摘要】 目的 探讨丹参酮ⅡA(TanⅡA)对谷氨酸(Glu)联合β淀粉样蛋白 2535 (Aβ2535)诱导PC12细胞、Meynert核(nbM)神经元损伤的保护作 用。方法 培养PC12细胞，用MTT法观察TanⅡA三个不同浓度(2.5、5.0、10 μmol/L)对Glu联合Aβ2535损伤PC12细胞的保护作用;运用激光扫描共聚 焦显微镜(LSCM)技术，检测急性分离nbM神经元在Glu联合Aβ诱导损伤时细 胞内钙离子浓度(〔Ca2+〕i)变化，及不同浓度TanⅡA对这种〔Ca2+〕i变化 的影响。结果 MTT检测结果表明，TanⅡA 各组细胞活力、损伤程度与单纯 Aβ+Glu损伤组相比有显著差异(P<0.05)，但无剂量依赖关系。Glu联合 Aβ2535损伤组〔Ca2+〕i比对照组显著升高(P<0.01);TanⅡA 各组 〔Ca2+〕i均较单独Glu联合Aβ2535处理组有显著降低(P<0.01)。结论 TanⅡA通过提高细胞活力、维持细胞内钙离子浓度稳态以对抗Glu联合 Aβ2535损伤作用。 【关键词】 阿尔茨海默病;β淀粉样蛋白;谷氨酸;Meynert基底核;PC12细胞 【Abstract】 Objective To explore the protection of TanshinoneⅡA (TanⅡA) on damage of PC12 cells and nucleus basalis of meynert (nbM) neurons induced by glutamate (Glu) and βamyloid protein 25～35 (Aβ25～35). Methods MTT assay was used to observe the protection of different dosages of Tan ⅡA(2.5, 5.0, 10 μmol/L)on cellular damage induced by Glu and Aβ2535. Laser scanning confocal microscopy (LSCM) technique was adopted to detect the change of the intracellular free calcium concentration (〔Ca2+〕i). Results There were significant differences on the cell survival and the extent of damage between the each Tan ⅡA group and Aβ+Glu group (P<0.05), but there wasn′t dosedependent manner. The level of 〔Ca2+〕i in Glu+Aβ25～35 group was significantly higher than that of control group (P<0.01). The level of 〔Ca2+〕i in each Tan ⅡA goup was significantly lower than that of Glu+Aβ2535 group(P<0.01). Conclusions Tan ⅡA can be against the cellular damage induced by Glu and Aβ2535 through increasing cell survival and sustaining the homeostasis of 〔Ca2+〕i. 【Key words】 Alzheimer′s disease (AD); βamyloid protein (Aβ); Glutamate (Glu); Nucleus basalis of Meynert (nbM); PC12 cells 目前西医 治疗 阿尔茨海默病(AD)主要是停留在缓解症状上。相比之下， 祖国医学在延缓衰老以及老年性疾病的防治方面有着丰富的理论和实践经验， 具有潜在的优势和广阔的开发前景。中药丹参〔1〕属于活血化瘀类中药，同时 具有养血安神功效。丹参酮ⅡA(TanⅡA)为丹参有效成分之一，为脂溶性、樱