云锦杜鹃NRT基因序列的生物信息学分析

云锦杜鹃NRT基因序列的生物信息学分析AbstractCamellia nitidissima is a Native evergreen shrub in China and has been wi

NRT 云锦杜鹃基因序列的生物信息学分析 Abstract Camellia nitidissima is aNative evergreen shrub in China and has been widely cultivated as an ornamental plant. As acharacteristic of its blooming time, it usually blooms earlier than other azaleas. In this paper, we analyzed the transcriptome of C. nitidissima by using next-generation sequencing technology, extracted and assembled the NRT gene sequence, and conducted aseries of bioinformatics analysis to explore the function and evolutionary relationship of NRT. Our results showed that NRT gene is ahomologous gene of nitrate transporter NRT1.3 identified in Arabidopsis thaliana, and NRT1.3 is essential for nitrate uptake and allocation in Arabidopsis. In addition, evolutionary analysis showed that NRT in C. nitidissima is closer to NRT1.3 in A. thaliana, and agene duplication event for NRT may occur after the divergence of the Camellia and Arabidopsis lineage. This study laid the foundation for further research on the function of NRT in C. nitidissima. Introduction Camellia nitidissima is amember of the Theaceae family, native to Guangdong, Guangxi, and Yunnan provinces in China. It is widely distributed in southeast China and is known for its beautiful flowers. Its flowering time is usually earlier than that of other azaleas, making it a popular ornamental plant. Nitrate is an essential nutrient for plant growth, affecting many developmental processes such as cell division and elongation, photosynthesis, and root growth. In addition, nitrate availability also affects plant resistance to biotic and abiotic stresses. Nitrate uptake and allocation in plants are mainly regulated by nitrate transporters, and the NRT gene family has been extensively studied in recent years. In Arabidopsis thaliana, NRT1.1 and NRT2.1 are known as high-affinity nitrate transporters, while NRT1.2 is alow-affinity nitrate transporter. NRT1.3 is known to function in nitrate uptake and allocation, and nrt1.3 mutants show impaired growth under low-nitrate conditions. Studying the function and evolutionary relationship of NRT in C. nitidissima is an important step to understand the molecular mechanism of nitrate uptake and allocation, which plays acritical role in plant growth and development. Methods