pcdna 3.1(+)图谱及序列

Plasmid Name           pcDNA3.1(+)  Alt Names              pcDNA3.1+, pcDNA3.1  Source/Vendor       

Plasmid Name pcDNA3.1(+) Alt Names pcDNA3.1+, pcDNA3.1 Source/Vendor Invitrogen Plasmid Type Mammalian Viral/Non-viral Nonviral Stable/Transient Transient Constitutive/Inducible Constitutive Promoter CMV Expression Level High Plasmid Size 5428 Sequencing Primer T7 Fwd Sequencing Primer Sequence 5'd[TAATACGACTCACTATAGGG]3' Bacterial Resistance Ampicilin Mammalian Selection G418, neo Notes Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori. Catalog Number V790-20 1GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATC 51 TGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTT 101 GGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAG 151 GCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCG 201 CTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGAC 251 TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA 301