药学毕业论文--高效液相色谱法测定八角莲中鬼臼毒素的含量

药学毕业论文--高效液相色谱法测定八角莲中鬼臼毒素的含量高效液相色谱法测定八角莲中鬼臼毒素的含量 【摘要】 目的建立高效液相色谱法测定八角莲中鬼臼毒素含量的方法。方法采用高效液相色谱法,色谱柱为Dia

药学毕业论文--高效液相色谱法测定八角莲中鬼臼毒素的 含量 高效液相色谱法测定八角莲中鬼臼毒素的含量 【摘要】 目的建立高效液相色谱法测定八角莲中鬼臼毒素含量的方 法。方法采用高效液相色谱法,色谱柱为Diamonsiltm C18柱;流 动相:甲醇-0.1 mol/L磷酸二氢钾水溶液，50?50，;流速:1.0 ml/min;检测波长:290 nm。结果 鬼臼毒素在0.047 69～0.476 9 mg/ml呈良好线性关系，r=0.999 6，,平均回收率为99.94%, RSD=1.71%，n=5，。结论 采用高效液相色谱法测定八角莲中鬼臼 毒素的含量,操作简便、准确,丏属性强。 【关键词】 高效液相色谱 八角莲 鬼臼毒素 Abstract:ObjectiveTo establish amethod of determining Podophllotoxin in Dysosma versipellis by HPLC.MethodsHigh Performance Liquid Chromatography was performed Diamonsil C18. The chromatography conditions were as follows: methanol -0.1mol/L monobasic potassium phosphate (50?50) as mobile phase, flow rate being 1.0ml/min and detecting wavelength was 290nm.ResultsA good linearity of Podophllotoxin was shown in range of 0.047 69～0.476 9 mg/ml (r=0.999 6), the average recovery was 99.94% and RSD 1 was 1.71%(n=5). ConclusionThe method can supply evidence for the quality evaluation of Dysosma versipellis. It is simple and accurate. Key words:HPLC; Dysosma versipellis; Podophllotoxin