上调NICD对人牙周膜干细胞增殖及迁移能力的影响

上调NICD对人牙周膜干细胞增殖及迁移能力的影响张大灵1 ,王源元1 ,刘莉2 ,周嫣1 ,李华3(1.广西壮族自治区人民医院口腔正畸科 广西南宁530021 ;2.桂林医学院附属口腔医院口腔科 广

上调对人牙周膜干细胞增殖及迁移能力的影响 NICD 112,13 张大灵,王源元,刘莉周嫣,李华 .广西壮族自治区人民医院口腔正畸科广西南宁桂林医学院附属口腔医院口腔科广西桂林.广西医科大学附属 (1530021;2.541000;3 口腔医院口腔颌面外科广西南宁 530021) ［摘要］目的：研究上调胞内区域对人牙周膜干细胞增殖及迁移能力的影响。方法：将 Notch(NotchIntraceIIuIarDomain,NICD) 细胞分为上调组、对照组及空白组，使用比色法检测细胞增殖能力，流式细胞仪检测细胞凋亡能力，采用细胞划痕实验检测细胞 MTT 迁移情况，并使用检测人牙周膜干细胞中信号通路蛋白、、、、 WesternblotWnt/B-cateninTGF-BBMP-2B-eatenLEFTCycIin ( 、、表达量。结果：对照组各时间点的人牙周膜干细胞增殖率、碱性磷酸酶活性、迁移、黏附个数、、 D1Caspase-3Bax>BeI-2)TGF-B 、、蛋白表达量均低于空白组，且凋亡率、蛋白表达量高于空白组，差异具有统计 BMP-23-eaten.LEFTCyclinDkBel-2Caspase-3.Bax 学意义。上调组各时间点人牙周膜干细胞增殖率、碱性磷酸酶活性、迁移、黏附个数、、、、 (P<0.05)TGF-0BMP-23-eaten.LEFTCycl 、蛋白表达量均高于空白组和对照组，且凋亡率、蛋白表达量均低于空白组和对照组，差异具有统计学意义 inD1Be1-2Caspase-3.Bax 。结论：上调基因能促进人牙周膜干细胞的增殖和迁移，其机制可能与通路有关。 (P<0.05)NICDWnt/3-eatenin ［关键词］胞内结构域；人牙周膜干细胞；增殖；凋亡；迁移；信号通路 Notch ［中图分类号］［文献标志码］［文章编号］ R781A1008-6455(2022)01-0097-05 EffectofUpregulationofNICDonProliferationandMigrationofHumanPeriodontal LigamentStemCells 11213 ZHANGDaling,WANGYuanyuan,LIULi,ZHOUYan,LIHua (1.DepartmentofOrthodontics,PeopledHospitalofGuangxiZhuangAutonomousRegion,Nanning530021,Guangxi,China; 2.DepartmentofStomatology,AffiliatedStomatologicalHospitalofGuilinMedicalUniversity,Guilin541000,Guangxi,China; 3.DepartmentofOralandMaxillofacialSurgery,HospitalofStomatology,GuangxiMedicalUniversity,Nanning 530021,Guangxi,China) Abstract:Objective ToinvestigatetheeffectofupregulatingNotchintracellulardomain(NICD)ontheproliferationandmigrationof Methods humanperiodontalligamentstemcells.NICDcellsweredividedintotheupregulationgroup,thecontrolgroupandtheblank group.MTTcolorimetrywasusedtodetectcellproliferation,flowcytometrywasusedtodetectcellapoptosis,cellmigrationwasdetected bycellscratchtest,andtheexpressionofWnt/p-cateninsignalingpathwayproteininhumanperiodontalligamentstemcellswasdetected Results 、 byWesternblot(TGF-0BMP-2,P-caten,LEF-1,CyclinDI,Caspase-3,Bax,Bcl-2).Theproliferationrate,alkalinephosphatase activity,migration,numberofadhesionandTGF-p,BMP-2,p-caten,LEF-1,CyclinDI,Bcl-2proteinexpressionofhumanperiodontal ligamentstemcellsinthecontrolgroupateachtimepointwerelowerthanthoseintheblankgroup,andtheapoptosisrate,theexpressionof Caspase-3andBaxproteinintheblankgroupwerehigherthanthoseintheblankgroup,thedifferenceswerestatisticallysignificant (P<0.05).Theproliferationrate,alkalinephosphataseactivity,migration,numberofadhesionandTGF-p,BMP-2,p-caten,LEF-1,Cyclin DI,Bcl-2proteinexpressionofhumanperiodontalligamentstemcellsintheupregulationgroupateachtimepointwerehigherthanthose intheblankgroupandthecontrolgroup(P<0.05).Theapoptosisrate,theexpressionofCaspase-3andBaxproteinintheupregulation Conclusion groupwerelowerthanthoseintheblankgroupandthecontrolgroup(P<0.05).UpregulationofNICDgenecanpromotethe Key proliferationandmigrationofhumanperiodontalligamentstemcells,anditsmechanismmayberelatedtoWnt/p-cateninpathway. words ： Notchintracellulardomain;humanperiodontalligamentstemcells;proliferation;apoptosis;migration;signalingpathways