南海鲈形目鱼类寄生异尖线虫幼虫的分子鉴定及亲缘关系分析

南海鲈形目鱼类寄生异尖线虫幼虫的分子鉴定及亲缘关系分析摘 要本文针对南海鲈形目鱼类寄生异尖线虫幼虫进行分子鉴定和亲缘关系分析，通过对线粒体cox1基因序列的比对和建树，确认该异尖线虫属于Myxospo

南海鲈形目鱼类寄生异尖线虫幼虫的分子鉴定及亲缘 关系分析 摘要 本文针对南海鲈形目鱼类寄生异尖线虫幼虫进行分子鉴定和亲缘关 cox1 系分析，通过对线粒体基因序列的比对和建树，确认该异尖线虫属 MyxosporeaCnidariaAuerbachia magdalensis 于门而非门，与已知的 Myxobolus spp. 和有较高的相似性，但与其它异尖线虫种类差异较大。 同时发现异尖线虫幼虫和南海鲈形目鱼类之间存在密切的寄生关系，这 对于南海鲈形目鱼类的养殖和管理具有一定的参考价值。 cox1 关键词：异尖线虫；鱼类寄生；分子鉴定；亲缘关系分析；基 因 Abstract This paper focuses on the molecular identification and phylogenetic analysis of the parasitic Myxosporean larvae of the South China Sea grouper order. By comparing and constructing the mitochondrial cox1 gene sequences, it was confirmed that this Myxosporean belongs to the Myxosporea phylum rather than the Cnidaria phylum. It shows high similarity with Auerbachia magdalensis and Myxobolus spp., but has significant differences from other Myxosporean species. At the same time, it was found that there is aclose parasitic relationship between the Myxosporean larvae and the South China Sea grouper order, which has acertain reference value for the cultivation and management of the South China Sea grouper order. Key words: Myxosporean; fish parasitism; molecular identification; phylogenetic analysis; cox1 gene 1. 引言 南海是我国最重要的渔业资源区之一，其丰富的海洋资源吸引着国 内外众多单位和个人进行调查和研究。其中，南海鲈形目鱼类以其肉质 鲜美，营养丰富，市场前景广阔而备受关注。但对于鱼类所承受的寄生