糖皮质激素致星形胶质细胞内钙浓度的快速升高

糖皮质激素致星形胶质细胞内钙浓度的快速升高 作者：李茂全，王艳艳，杨书，陈朝琼，杨晓虹，余争平 【关键词】 应激；糖皮质激素；星形细胞；钙 Rapid elevation of intr

15 第页共页 糖皮质激素致星形胶质细胞内钙浓度的快速升高 作者：李茂全，王艳艳，杨书，陈朝琼，杨晓虹，余争平【关 键词】应激；糖皮质激素；星形细胞；钙 Rapidelevationofintracellularfreecalcium concentrationinastrocytesaftertreatedbyglucocorticoid 【Abstract】AIM:Toexplorethemechanismsof glucocorticoid(GC)leadingtotheelevationof intracellularfreecalciumconcentration(［Ca2+］i)in astrocytesinearlyeffectphase.METHODS:Acellculture modelofneonatalWistarrathippocampalastrocyteswas used.Afterexposedtocorticosterone(CORT)(1μmol/L), NmethylDaspartate(NMDA),MK801andBSACORT,respectively, changesof［Ca2+］iinastrocyteswereobservedbylaser confocalmicroscope.RESULTS:CORTinducedrapidelevation ofintracellularCa2+fluorescenceintensitywithin10min （177.1±3.8，P95%.提示所分离的星形胶质细胞纯度高（图1）.图 1间接免疫荧光法鉴定培养的海马星形胶质细胞纯度×200