siRNA干扰MagT1对原代大鼠心肌细胞影响的初步研究

siRNA干扰MagT1对原代大鼠心肌细胞影响的初步研究摘要：MagT1是一种新发现的离子通道，在心肌细胞中起着重要作用。本研究利用siRNA技术干扰MagT1基因表达，旨在探究其对原代大鼠心肌细胞的

siRNAMagT1 干扰对原代大鼠心肌细胞影响的初步 研究 摘要：MagT1是一种新发现的离子通道，在心肌细胞中起着重要作 用。本研究利用siRNA技术干扰MagT1基因表达，旨在探究其对原代 大鼠心肌细胞的影响。结果表明，MagT1基因的siRNA干扰可以有效 地抑制MagT1的表达，降低心肌细胞内钙离子浓度，并且影响心肌细胞 的收缩力和肌原纤维的形态。这些发现为进一步研究MagT1在心肌细胞 中的作用提供了基础。 关键词：MagT1；siRNA；心肌细胞；钙离子；收缩力 Abstract:MagT1isanewlydiscoveredionchannelthatplays animportantroleincardiaccells.Thisstudyaimedtoinvestigate theeffectofMagT1geneexpressioninterferencebysiRNAon primaryratcardiaccells.TheresultsshowedthatsiRNA interferenceofMagT1geneexpressioneffectivelyinhibitedthe expressionofMagT1,reducedtheconcentrationofcalciumionsin cardiaccells,andaffectedthecontractilityandmorphologyof cardiacmyofibers.Thesefindingsprovideabasisforfurther researchontheroleofMagT1incardiaccells. Keywords:MagT1;siRNA;cardiaccells;calciumions; contractility Introduction Theheartisanessentialorganthatpumpsbloodthroughout thebody.Itismadeupofcardiaccells,whichareresponsiblefor thegenerationandpropagationofelectricalimpulsesthatcontrol therhythmandcontractilityoftheheart.Calciumionsplaya crucialroleinthefunctionofcardiaccells,includingtheregulation ofcontractilityandelectricalsignaling.MagT1isanewly discoveredionchannelthathasbeenshowntoplayanimportant roleinthetransportofmagnesiumionsandcalciumionsacross thecellmembraneinvariouscelltypes,includingcardiaccells. SiRNAisapowerfultoolforstudyinggeneexpressionand function.ItworksbydegradingthemRNAofthetargetedgene, preventingitstranslationintoprotein.Inthepresentstudy,we